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An Introduction to The Low Speed Centrifugation Concept

Updated: Jan 20

Definition: A separation method where the components of a sample are separated based on their density in a centrifuge according to the centrifugal force they experience.



The low‐speed centrifugation concept (LSCC) indicates that reducing the relevant centrifugation force (RCF) yields the highest number of leukocytes and platelets. Platelets play a critical role in tissue repair and regeneration, they regulate fundamental mechanisms involved in the healing process including cellular migration, proliferation, and angiogenesis. Leukocytes are the main protagonists in wound healing and the regeneration process. Their presence in autologous therapy increases the regeneration capacity.

“One of the areas that has led to great confusion over the years is that RCF values have been calculated at various regions along a centrifugation tube. For these reasons, it is important to have a basic understanding of RCF values .”

It is recommended that only approved devices and centrifuges should be used to prepare a specific type of PRP or i-PRF.


Cell Rich Plasma


A recent analysis comparing hard spin and soft spin parameters confirmed the hypothesis that LSCC yields greater cell count than hard spin centrifugation.

The high concentrations of cells are important, as the white blood cell count in PRP samples has frequently been ignored, being considered insignificant. The lack of standardization of PRP preparation for clinical use has contributed at least in part to the varying clinical efficacy in PRP use.

i-PRF offers simplicity, consistency and cell rich plasma compared to standard PRP.


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